2W93
Crystal structure of the Saccharomyces cerevisiae pyruvate decarboxylase variant E477Q in complex with the surrogate pyruvamide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | PYRUVATE DECARBOXYLASE ISOZYME 1 | polymer | 563 | 61571.0 | 4 | UniProt (P06169) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | PYRUVATE DECARBOXYLASE |
| 2 | E, I, M, Q (A, B, C, D) | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 4 | Chemie (TPP) | |||
| 3 | F, J, N, R (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 4 | G, H, K, L, O... (A, B, C, D) | (1S,2S)-1-amino-1,2-dihydroxypropan-1-olate | non-polymer | 106.1 | 8 | Chemie (PY0) | |||
| 5 | U, V, W, X (A, B, C, D) | water | water | 18.0 | 1413 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 563 (UniProt: P06169)
| PDB | External Database | Details |
|---|---|---|
| Ser 106 | Ala 106 | conflict |
| Asn 336 | Thr 336 | conflict |
| Gln 477 | Glu 477 | engineered mutation |
| Val 538 | Ile 538 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 246284.1 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 2647.3 | |
| All* | Total formula weight | 248931.4 |
