2VXL
Screening a Limited Structure-based Library Identifies UDP-GalNAc- Specific Mutants of alpha-1,3 Galactosyltransferase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | N-ACETYLLACTOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE | polymer | 277 | 32560.5 | 1 | UniProt (P14769) Pfam (PF03414) | BOS TAURUS (BOVINE) | GALACTOSYLTRANSFERASE, UDP-GALACTOSE\: BETA-D-GALACTOSYL-1,4-N-ACETYL-D-GLUCOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE, ALPHA-1,3 GALACTOSYLTRANSFERASE |
| 2 | B (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) PubChem (27854) | |||
| 3 | C (A) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 1 | Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) | |||
| 4 | D (A) | water | water | 18.0 | 43 | Chemie (HOH) |
Sequence modifications
A: 82 - 358 (UniProt: P14769)
| PDB | External Database | Details |
|---|---|---|
| Ala 280 | His 280 | engineered mutation |
| Gly 281 | Ala 281 | engineered mutation |
| Gly 282 | Ala 282 | engineered mutation |
| Leu 283 | Ile 283 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 32560.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 459.1 | |
| All* | Total formula weight | 33019.6 |
