2VXL
Screening a Limited Structure-based Library Identifies UDP-GalNAc- Specific Mutants of alpha-1,3 Galactosyltransferase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | N-ACETYLLACTOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE | polymer | 277 | 32560.5 | 1 | UniProt (P14769) Pfam (PF03414) | BOS TAURUS (BOVINE) | GALACTOSYLTRANSFERASE, UDP-GALACTOSE\: BETA-D-GALACTOSYL-1,4-N-ACETYL-D-GLUCOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE, ALPHA-1,3 GALACTOSYLTRANSFERASE |
| 2 | B (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 3 | C (A) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 1 | Chemie (UDP) | |||
| 4 | D (A) | water | water | 18.0 | 43 | Chemie (HOH) |
Sequence modifications
A: 82 - 358 (UniProt: P14769)
| PDB | External Database | Details |
|---|---|---|
| Ala 280 | His 280 | engineered mutation |
| Gly 281 | Ala 281 | engineered mutation |
| Gly 282 | Ala 282 | engineered mutation |
| Leu 283 | Ile 283 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 32560.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 459.1 | |
| All* | Total formula weight | 33019.6 |
