2VK8
Crystal structure of the Saccharomyces cerevisiae pyruvate decarboxylase variant E477Q in complex with its substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | PYRUVATE DECARBOXYLASE ISOZYME 1 | polymer | 563 | 61586.0 | 4 | UniProt (P06169) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | PYRUVATE DECARBOXYLASE |
| 2 | E, H, K, N (A, B, C, D) | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 4 | Chemie (TPP) | |||
| 3 | F, I, L, O (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 4 | G, J, M, P (A, B, C, D) | (2S)-2-HYDROXYPROPANOIC ACID | non-polymer | 90.1 | 4 | Chemie (2OP) | |||
| 5 | Q, R, S, T (A, B, C, D) | water | water | 18.0 | 1818 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 563 (UniProt: P06169)
| PDB | External Database | Details |
|---|---|---|
| Gln 477 | Glu 477 | engineered mutation |
| Val 206 | Ala 206 | conflict |
| Asp 208 | Val 208 | conflict |
| Val 538 | Ile 538 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 246344.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2158.8 | |
| All* | Total formula weight | 248503.0 |
