2VBJ

Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ENDONUCLEASE I-CREI	polymer	152	17566.2	1	UniProt (P05725) Pfam (PF00961)	CHLAMYDOMONAS REINHARDTII	AMEL3-4-CA, 23S RRNA INTRON PROTEIN
2	B (B)	DNA ENDONUCLEASE I-CREI	polymer	152	17454.1	1	UniProt (P05725) Pfam (PF00961)	CHLAMYDOMONAS REINHARDTII	AMEL3-4-CA, 23S RRNA INTRON PROTEIN
3	C (C)	5'-D(*TP*CP*TP*GP*CP*CP*TP*TP*TP*TP *TP*TP*GP*AP*AP*GP*GP*AP*TP*CP*CP*TP*AP*A)-3'	polymer	24	7325.7	1		synthetic construct
4	D (E)	5'-D(*TP*TP*AP*GP*GP*AP*TP*CP*CP*TP *TP*CP*AP*AP*AP*AP*AP*AP*GP*GP*CP*AP*GP*A)-3'	polymer	24	7410.8	1		synthetic construct
5	E, F (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	G, H, I, J (A, B, C, E)	water	water		18.0	330	Chemie (HOH)

Sequence modifications

A: 2 - 153 (UniProt: P05725)

PDB	External Database	Details
Glu 28	Lys 28	conflict
Arg 38	Gln 38	conflict
Lys 40	Ser 40	conflict
Thr 42	Ala 42	conflict
Lys 44	Gln 44	conflict
Glu 70	Arg 70	conflict
Asn 75	Asp 75	conflict
Arg 85	His 85	conflict
Thr 109	Ile 109	conflict
Glu 110	Trp 110	conflict
Gln 111	Arg 111	conflict

B: 2 - 153 (UniProt: P05725)

PDB	External Database	Details
Ala 19	Gly 19	conflict
Glu 28	Lys 28	conflict
Ser 33	Tyr 33	conflict
Arg 38	Gln 38	conflict
Lys 40	Ser 40	conflict
Thr 42	Ala 42	conflict
Ser 70	Arg 70	conflict
Asn 75	Asp 75	conflict
Glu 110	Trp 110	conflict
Gln 111	Arg 111	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49756.8
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	49837.0

*Water molecules are not included.