2UZ4

HhaI DNA methyltransferase R165N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MODIFICATION METHYLASE HHAI	polymer	327	36999.1	1	UniProt (P05102)	HAEMOPHILUS PARAHAEMOLYTICUS	HHAI DNA METHYLTRANSFERASE, CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI, M.HHAI
2	B (C)	5'-D(*TP*GP*GP*AP*TP*GP*5CMP*GP*CP*TP*GP *AP*C)-3'	polymer	13	4021.6	1			DNA OLIGONUCLEOTIDE
3	C (D)	5'-D(*GP*TP*CP*AP*GP*CP*GP*CP*AP*TP *CP*C)-3'	polymer	12	3623.4	1			DNA OLIGONUCLEOTIDE
4	D (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (25210495) PubChem (13792) PubChem (177678424) PubChem (44361184) PubChem (21125603) PubChem (46936206) PubChem (50989525) PubChem (25203782) PubChem (51380914) PubChem (52224398) PubChem (189149) PubChem (57544532) PubChem (439155) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (52224400) PubChem (5284331) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (71170554) PubChem (13231890) PubChem (164157219) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525)
5	E, F, G, H (A)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
6	I, J (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
7	K, L, M (A, C, D)	water	water		18.0	142	Chemie (HOH)

Sequence modifications

A: 1 - 327 (UniProt: P05102)

PDB	External Database	Details
Asn 165	Arg 165	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44644.1
Non-Polymers*	Number of molecules	7
	Total formula weight	952.9
All*	Total formula weight	45597.0

*Water molecules are not included.