2UVW

Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA POLYMERASE IV	polymer	358	41058.7	1	UniProt (Q97W02) Pfam (PF00817) Pfam (PF11798) Pfam (PF11799) In PDB	SULFOLOBUS SOLFATARICUS	POL IV
2	P	5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP *TP*TP*CP*A)-3'	polymer	14	4409.9	1			14-MER PRIMER
3	T	5'-D(*TP*CP*AP*CP*8OGP*GP*AP*AP*TP*CP *CP*TP*TP*CP*CP*CP*CP*C)-3'	polymer	18	5388.5	1			18-MER TEMPLATE
4	A	2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (DGT)
5	A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
6		water	water		18.0	224	Chemie (HOH)

Sequence modifications

A: -5 - 0 (PDB: 2UVW)
A: 1 - 352 (UniProt: Q97W02)

PDB	External Database	Details
Glu 332	Arg 332	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	50857.0
Non-Polymers*	Number of molecules	4
	Total formula weight	627.4
All*	Total formula weight	51484.4

*Water molecules are not included.