2RHK

Crystal structure of influenza A NS1A protein in complex with F2F3 fragment of human cellular factor CPSF30, Northeast Structural Genomics Targets OR8C and HR6309A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Non-structural protein 1	polymer	140	15964.4	2	UniProt (P03495) Pfam (PF00600)	Influenza A Virus	NS1, NS1A
2	C, D (C, D)	Cleavage and polyadenylation specificity factor subunit 4	polymer	72	8570.8	2	UniProt (O95639) Pfam (PF15663)	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 30 kDa subunit, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1, Neb-1, No arches homolog, F2F3 Zinc-binding domains
3	E, F, G, H, K (A, C)	NITRATE ION	non-polymer		62.0	5	Chemie (NO3)
4	I, J, L, M (C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	N (D)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
6	O, P, Q, R (A, B, C, D)	water	water		18.0	185	Chemie (HOH)

Sequence modifications

A, B: 85 - 215 (UniProt: P03495)

PDB	External Database	Details
Met 84	-	initiating methionine
Leu 216	-	expression tag
Glu 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag

C, D: 61 - 121 (UniProt: O95639)

PDB	External Database	Details
Met 50	-	expression tag
Gly 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag
Ser 58	-	expression tag
His 59	-	expression tag
Met 60	-	expression tag
Ser 94	Pro 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49070.4
Non-Polymers*	Number of molecules	10
	Total formula weight	693.8
All*	Total formula weight	49764.2

*Water molecules are not included.