2RE8
Glutaminyl-tRNA synthetase mutant C229R with bound analog 5'-O-[N-(L-GLUTAMYL)-SULFAMOYL]ADENOSINE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Glutamine tRNA | polymer | 75 | 24099.3 | 1 | |||
| 2 | B (A) | Glutaminyl-tRNA synthetase | polymer | 556 | 64019.3 | 1 | UniProt (P00962) Pfam (PF00749) Pfam (PF03950) Pfam (PF20974) | Escherichia coli | Glutamine--tRNA ligase, GlnRS |
| 3 | C, D (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | E (A) | O5'-(L-GLUTAMYL-SULFAMOYL)-ADENOSINE | non-polymer | 475.4 | 1 | Chemie (GSU) | |||
| 5 | F, G (B, A) | water | water | 18.0 | 137 | Chemie (HOH) |
Sequence modifications
A: 0 - 547 (UniProt: P00962)
| PDB | External Database | Details |
|---|---|---|
| Arg 229 | Cys 230 | engineered mutation |
| Leu 548 | - | expression tag |
| Glu 549 | - | expression tag |
| His 550 | - | expression tag |
| His 551 | - | expression tag |
| His 552 | - | expression tag |
| His 553 | - | expression tag |
| His 554 | - | expression tag |
| His 555 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 88118.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 667.6 | |
| All* | Total formula weight | 88786.2 |
