2PYL
Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (X) | 5'-d(GACTGCTTAC(2DA))-3' | polymer | 11 | 3317.2 | 1 | |||
| 2 | B (Y) | 5'-d(CTGACGAATGTACA)-3' | polymer | 14 | 4288.8 | 1 | |||
| 3 | C (A) | DNA polymerase | polymer | 575 | 66720.9 | 1 | UniProt (P03680) Pfam (PF03175) | Bacillus phage phi29 | Early protein GP2 |
| 4 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
| 6 | G, H, I, J, K... (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 7 | M, N, O (X, Y, A) | water | water | 18.0 | 357 | Chemie (HOH) |
Sequence modifications
A: 1 - 575 (UniProt: P03680)
| PDB | External Database | Details |
|---|---|---|
| Ala 12 | Asp 12 | engineered mutation |
| Ala 66 | Asp 66 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 74326.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 903.2 | |
| All* | Total formula weight | 75230.1 |
