2PUJ
Crystal Structure of the S112A/H265A double mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase | polymer | 286 | 31985.6 | 1 | UniProt (P47229) Pfam (PF00561) | Burkholderia xenovorans | |
| 2 | B (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 3 | C (A) | MALONATE ION | non-polymer | 102.0 | 1 | Chemie (MLI) | |||
| 4 | D (A) | (2E,4E)-2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID | non-polymer | 218.2 | 1 | Chemie (HPZ) | |||
| 5 | E (A) | water | water | 18.0 | 153 | Chemie (HOH) |
Sequence modifications
A: 1 - 286 (UniProt: P47229)
| PDB | External Database | Details |
|---|---|---|
| Ala 112 | Ser 112 | engineered mutation |
| Ala 265 | His 265 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31985.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 343.2 | |
| All* | Total formula weight | 32328.8 |
