2PTR

Crystal structure of Escherichia coli adenylosuccinate lyase mutant H171A with bound adenylosuccinate substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Adenylosuccinate lyase	polymer	462	52369.4	2	UniProt (P0AB89) Pfam (PF00206) Pfam (PF08328) In PDB	Escherichia coli	Adenylosuccinate, ASL
2	B	2-[9-(3,4-DIHYDROXY-5-PHOSPHONOOXYMETHYL-TETRAHYDRO-FURAN-2-YL)-9H-PURIN-6-YLAMINO]-SUCCINIC ACID	non-polymer		463.3	2	Chemie (2SA)
3		water	water		18.0	610	Chemie (HOH)

Sequence modifications

A, B: 1 - 456 (UniProt: P0AB89)

PDB	External Database	Details
Leu 154	Ile 154	conflict
Ala 171	His 171	engineered mutation
His 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	104738.9
Non-Polymers*	Number of molecules	2
	Total formula weight	926.6
All*	Total formula weight	105665.4

*Water molecules are not included.