2PTQ

Crystal structure of Escherichia coli adenylosuccinate lyase mutant H171N with bound AMP and fumarate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Adenylosuccinate lyase	polymer	462	52412.4	2	UniProt (P0AB89) Pfam (PF00206) Pfam (PF08328)	Escherichia coli	Adenylosuccinate, ASL
2	C, E (A, B)	FUMARIC ACID	non-polymer		116.1	2	Chemie (FUM)
3	D, F (A, B)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
4	G, H (A, B)	water	water		18.0	650	Chemie (HOH)

Sequence modifications

A, B: 1 - 456 (UniProt: P0AB89)

PDB	External Database	Details
Leu 154	Ile 154	conflict
Asn 171	His 171	engineered mutation
His 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	104824.9
Non-Polymers*	Number of molecules	4
	Total formula weight	926.6
All*	Total formula weight	105751.5

*Water molecules are not included.