2PTQ
Crystal structure of Escherichia coli adenylosuccinate lyase mutant H171N with bound AMP and fumarate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Adenylosuccinate lyase | polymer | 462 | 52412.4 | 2 | UniProt (P0AB89) Pfam (PF00206) Pfam (PF08328) | Escherichia coli | Adenylosuccinate, ASL |
| 2 | C, E (A, B) | FUMARIC ACID | non-polymer | 116.1 | 2 | Chemie (FUM) | |||
| 3 | D, F (A, B) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 2 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40459109) PubChem (46875232) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (177678969) PubChem (6083) PubChem (46874558) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (12358354) PubChem (57879893) PubChem (57879902) PubChem (224) PubChem (122197642) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 650 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 456 (UniProt: P0AB89)
| PDB | External Database | Details |
|---|---|---|
| Leu 154 | Ile 154 | conflict |
| Asn 171 | His 171 | engineered mutation |
| His 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
| His 461 | - | expression tag |
| His 462 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 104824.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 926.6 | |
| All* | Total formula weight | 105751.5 |
