2PSE

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Renilla-luciferin 2-monooxygenase	polymer	318	37000.2	1	UniProt (P27652) Pfam (PF00561) In PDB	Renilla reniformis	Renilla-type luciferase
2	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
3		water	water		18.0	76	Chemie (HOH)

Sequence modifications

A: 3 - 311 (UniProt: P27652)

PDB	External Database	Details
Ala 2	-	expression tag
Thr 55	Ala 55	engineered mutation
Ala 124	Cys 124	engineered mutation
Ala 130	Ser 130	engineered mutation
Arg 136	Lys 136	engineered mutation
Met 143	Ala 143	engineered mutation
Val 185	Met 185	engineered mutation
Leu 253	Met 253	engineered mutation
Leu 287	Ser 287	engineered mutation
Val 312	-	expression tag
Asp 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37000.2
Non-Polymers*	Number of molecules	1
	Total formula weight	69.1
All*	Total formula weight	37069.3

*Water molecules are not included.