2PPF
Reduced mutant D98N of AfNiR exposed to nitric oxide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Copper-containing nitrite reductase | polymer | 341 | 36877.7 | 3 | UniProt (P38501) Pfam (PF07732) Pfam (PF00394) | Alcaligenes faecalis | Cu-NIR |
| 2 | D, I, K, R, T (A, B, C) | COPPER (I) ION | non-polymer | 63.5 | 5 | Chemie (CU1) | |||
| 3 | E, J, S (A, B, C) | COPPER (II) ION | non-polymer | 63.5 | 3 | Chemie (CU) | |||
| 4 | F, L, M, N, O... (A, B, C) | ACETATE ION | non-polymer | 59.0 | 8 | Chemie (ACT) | |||
| 5 | G, H, P, X (A, B, C) | NITRIC OXIDE | non-polymer | 30.0 | 4 | Chemie (NO) | |||
| 6 | Q (B) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 7 | AA, Y, Z (C, A, B) | water | water | 18.0 | 998 | Chemie (HOH) |
Sequence modifications
A, B, C: 4 - 340 (UniProt: P38501)
| PDB | External Database | Details |
|---|---|---|
| Asn 98 | Asp 134 | engineered mutation |
| Ile 341 | - | expression tag |
| Glu 342 | - | expression tag |
| Gly 343 | - | expression tag |
| Arg 344 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 110633.0 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 1222.9 | |
| All* | Total formula weight | 111855.9 |
