2OSY
Endo-glycoceramidase II from Rhodococcus sp.: Lactosyl-Enzyme Intermediate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Endoglycoceramidase II | polymer | 481 | 52064.5 | 2 | UniProt (O33853) | Rhodococcus sp. | |
| 2 | C, D (C, D) | beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900008) GlyTouCan (G88362QR) | alpha-lactose | ||
| 3 | E, F (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 301 | Chemie (HOH) |
Sequence modifications
A, B: 31 - 490 (UniProt: O33853)
| PDB | External Database | Details |
|---|---|---|
| Met 10 | - | cloning artifact |
| Gly 11 | - | cloning artifact |
| Ser 12 | - | cloning artifact |
| Ser 13 | - | cloning artifact |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| Ser 20 | - | cloning artifact |
| Ser 21 | - | cloning artifact |
| Gly 22 | - | cloning artifact |
| Leu 23 | - | cloning artifact |
| Val 24 | - | cloning artifact |
| Pro 25 | - | cloning artifact |
| Arg 26 | - | cloning artifact |
| Gly 27 | - | cloning artifact |
| Ser 28 | - | cloning artifact |
| His 29 | - | cloning artifact |
| Met 30 | - | cloning artifact |
| Ala 233 | Glu 233 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 104129.0 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 46.0 | |
| All* | Total formula weight | 104859.6 |
