2OQ4
Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli (E2Q) in complex with AP-site containing DNA substrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C (C, E) | 5'-D(*G*GP*CP*TP*TP*CP*AP*TP*CP*CP*TP*GP*G)-3' | polymer | 13 | 3958.6 | 2 | |||
2 | B, D (D, F) | 5'-D(*C*CP*AP*GP*GP*AP*(PED)P*GP*AP*AP*GP*CP*C)-3' | polymer | 13 | 3863.5 | 2 | |||
3 | E, F (A, B) | Endonuclease VIII | polymer | 262 | 29814.0 | 2 | UniProt (P50465) Pfam (PF01149) Pfam (PF06831) Pfam (PF06827) | Escherichia coli | DNA glycosylase/AP lyase Nei, DNA-apurinic or apyrimidinic site, lyase Nei |
4 | G, M (F, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
5 | H, J (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
6 | I, K, L (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
7 | N, O, P, Q, R... (C, D, E, F, A...) | water | water | 18.0 | 93 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 262 (UniProt: P50465)
PDB | External Database | Details |
---|---|---|
Gln 2 | Glu 3 | engineered mutation |
Thr 34 | Pro 35 | SEE REMARK 999 |
Arg 112 | Thr 113 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 75272.2 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 465.0 | |
All* | Total formula weight | 75737.2 |