2OCF
Human estrogen receptor alpha ligand-binding domain in complex with estradiol and the E2#23 FN3 monobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Estrogen receptor | polymer | 298 | 33679.2 | 1 | UniProt (P03372) Pfam (PF00104) Pfam (PF12743) | Homo sapiens (human) | ER, Estradiol receptor, ER-alpha |
| 2 | B (D) | Fibronectin | polymer | 121 | 13245.8 | 1 | UniProt (P02751) Pfam (PF00041) | Homo sapiens (human) | FN, Cold-insoluble globulin, CIG |
| 3 | C (A) | ESTRADIOL | non-polymer | 272.4 | 1 | Chemie (EST) | |||
| 4 | D, E (A, D) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A: 298 - 595 (UniProt: P03372)
D: 1 - 94 (UniProt: P02751)
| PDB | External Database | Details |
|---|---|---|
| Cme 381 | Cys 381 | modified residue |
| Cme 417 | Cys 417 | modified residue |
| Ser 537 | Tyr 537 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -26 | - | insertion |
| Lys -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Lys -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Thr 6 | Arg 1452 | engineered mutation |
| Lys 7 | Asp 1453 | engineered mutation |
| Leu 78 | Arg 1524 | engineered mutation |
| Arg 79 | Gly 1525 | engineered mutation |
| Leu 80 | Asp 1526 | engineered mutation |
| Met 81 | Ser 1527 | engineered mutation |
| Leu 82 | Pro 1528 | engineered mutation |
| Gly 84 | Ser 1530 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 46925.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 272.4 | |
| All* | Total formula weight | 47197.4 |
