2OCF
Human estrogen receptor alpha ligand-binding domain in complex with estradiol and the E2#23 FN3 monobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Estrogen receptor | polymer | 298 | 33679.2 | 1 | UniProt (P03372) Pfam (PF00104) Pfam (PF12743) | Homo sapiens (human) | ER, Estradiol receptor, ER-alpha |
| 2 | B (D) | Fibronectin | polymer | 121 | 13245.8 | 1 | UniProt (P02751) Pfam (PF00041) | Homo sapiens (human) | FN, Cold-insoluble globulin, CIG |
| 3 | C (A) | ESTRADIOL | non-polymer | 272.4 | 1 | Chemie (EST) PubChem (16757676) PubChem (177015577) PubChem (1807200) PubChem (43912931) PubChem (1807201) PubChem (44284023) PubChem (46781508) PubChem (5317222) PubChem (54403267) PubChem (56624791) PubChem (57041922) PubChem (12313788) PubChem (162947701) PubChem (58650893) PubChem (59084698) PubChem (166849971) PubChem (68014581) PubChem (68209947) PubChem (70220248) PubChem (70864205) PubChem (91740223) PubChem (101691329) PubChem (118548795) PubChem (450) PubChem (5757) PubChem (5998) PubChem (10596) PubChem (14880) PubChem (68570) PubChem (667480) PubChem (667515) PubChem (721867) PubChem (928284) PubChem (1258565) PubChem (5317221) PubChem (5745505) PubChem (6328313) PubChem (6432565) PubChem (6560352) PubChem (6603846) PubChem (6918968) PubChem (6919041) PubChem (7000012) PubChem (7004689) PubChem (7048584) PubChem (7048670) PubChem (7061189) PubChem (7067800) PubChem (126969992) PubChem (129010410) PubChem (9795489) PubChem (9811784) PubChem (9903595) PubChem (9938293) PubChem (129317336) PubChem (11869602) PubChem (11870431) PubChem (130307439) PubChem (130307440) PubChem (12313787) PubChem (12313791) PubChem (12313794) PubChem (143489158) PubChem (144605119) PubChem (145157829) PubChem (145610824) PubChem (154109755) PubChem (172873304) | |||
| 4 | D, E (A, D) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A: 298 - 595 (UniProt: P03372)
D: 1 - 94 (UniProt: P02751)
| PDB | External Database | Details |
|---|---|---|
| Cme 381 | Cys 381 | modified residue |
| Cme 417 | Cys 417 | modified residue |
| Ser 537 | Tyr 537 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -26 | - | insertion |
| Lys -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Asp -16 | - | expression tag |
| Tyr -15 | - | expression tag |
| Lys -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Lys -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Thr 6 | Arg 1452 | engineered mutation |
| Lys 7 | Asp 1453 | engineered mutation |
| Leu 78 | Arg 1524 | engineered mutation |
| Arg 79 | Gly 1525 | engineered mutation |
| Leu 80 | Asp 1526 | engineered mutation |
| Met 81 | Ser 1527 | engineered mutation |
| Leu 82 | Pro 1528 | engineered mutation |
| Gly 84 | Ser 1530 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 46925.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 272.4 | |
| All* | Total formula weight | 47197.4 |
