2O8E

human MutSalpha (MSH2/MSH6) bound to a G T mispair, with ADP bound to MSH2 only

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	5'-D(*GP*AP*AP*CP*CP*GP*CP*GP*GP*GP*CP*TP*AP*GP*G)-3'	polymer	15	4660.0	1
2	F	5'-D(*CP*CP*TP*AP*GP*CP*CP*TP*GP*CP*GP*GP*TP*TP*C)-3'	polymer	15	4536.9	1
3	A	DNA mismatch repair protein Msh2	polymer	934	104785.8	1	UniProt (P43246) Pfam (PF01624) Pfam (PF05188) Pfam (PF05192) Pfam (PF05190) Pfam (PF00488) In PDB	Homo sapiens (human)	MutS protein homolog 2
4	B	DNA mismatch repair protein MSH6	polymer	1022	116056.7	1	UniProt (P52701) Pfam (PF00488) Pfam (PF01624) Pfam (PF05188) Pfam (PF05192) Pfam (PF05190) In PDB	Homo sapiens (human)	MutS-alpha 160 kDa subunit, G/T mismatch-binding protein, GTBP, GTMBP, p160
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
7		water	water		18.0	10	Chemie (HOH)

Sequence modifications

A: 1 - 934 (UniProt: P43246)

PDB	External Database	Details
Ala 42	Phe 42	engineered mutation

B: 341 - 1360 (UniProt: P52701)

PDB	External Database	Details
Met 339	-	initiating methionine
Gly 340	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	230039.4
Non-Polymers*	Number of molecules	2
	Total formula weight	451.5
All*	Total formula weight	230490.9

*Water molecules are not included.