2O3B

Crystal structure complex of Nuclease A (NucA) with intra-cellular inhibitor NuiA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nuclease	polymer	241	26375.4	1	UniProt (P38446) Pfam (PF01223) In PDB	Nostoc sp.	Endonuclease
2	B	Sugar-non-specific nuclease inhibitor	polymer	136	15133.8	1	UniProt (Q7A260) Pfam (PF07924) In PDB	Nostoc sp.
3	A	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6		water	water		18.0	227	Chemie (HOH)

Sequence modifications

A: 35 - 274 (UniProt: P38446)

PDB	External Database	Details
Gly 34	-	cloning artifact
Ala 121	Asp 121	engineered mutation

B: 2 - 135 (UniProt: Q7A260)

PDB	External Database	Details
Gly 0	-	cloning artifact
Ser 1	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	41509.2
Non-Polymers*	Number of molecules	5
	Total formula weight	532.2
All*	Total formula weight	42041.4

*Water molecules are not included.