2O18

Crystal structure of a Thiamine biosynthesis lipoprotein apbE, NorthEast Strcutural Genomics target ER559

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Thiamine biosynthesis lipoprotein apbE	polymer	340	38166.3	4	UniProt (P0AB85) Pfam (PF02424) In PDB	Escherichia coli
2	A, B, C, D	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3		water	water		18.0	203	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 332 (UniProt: P0AB85)

PDB	External Database	Details
Mse 1	-	initiating methionine
Mse 23	Met 42	modified residue
Mse 67	Met 86	modified residue
Mse 84	Met 103	modified residue
Mse 102	Met 121	modified residue
Mse 133	Met 152	modified residue
Mse 180	Met 199	modified residue
Mse 201	Met 220	modified residue
Mse 287	Met 306	modified residue
Mse 307	Met 326	modified residue
Mse 319	Met 338	modified residue
Leu 333	-	cloning artifact
Glu 334	-	cloning artifact
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	152665.1
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	152825.4

*Water molecules are not included.