2NZ4
Structural investigation of the GlmS ribozyme bound to its catalytic cofactor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (E, F, G, H) | substrate strand RNA 13-mer | polymer | 13 | 4177.6 | 4 | |||
| 2 | B, D, F, H (P, Q, R, S) | GlmS ribozyme | polymer | 141 | 45624.9 | 4 | |||
| 3 | I, J, K, L (A, B, C, D) | U1 Small Nuclear Ribonucleoprotein A | polymer | 94 | 10941.8 | 4 | UniProt (P09012) Pfam (PF00076) | Homo sapiens (human) | U1 snRNP protein A; U1A protein; U1-A |
| 4 | BA, M, R, W (H, P, F, G) | 2-amino-2-deoxy-6-O-phosphono-alpha-D-glucopyranose | non-polymer | 259.2 | 4 | Chemie (GLP) | |||
| 5 | AA, CA, DA, EA, FA... (R, H, S, P, F...) | MAGNESIUM ION | non-polymer | 24.3 | 16 | Chemie (MG) | |||
| 6 | GA, HA, IA, JA, KA... (E, P, F, Q, G...) | water | water | 18.0 | 206 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 5 - 98 (UniProt: P09012)
| PDB | External Database | Details |
|---|---|---|
| His 31 | Tyr 30 | engineered mutation |
| Arg 36 | Gln 35 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 242977.1 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 1425.5 | |
| All* | Total formula weight | 244402.5 |
