2NNW

Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	NOP5/NOP56 related protein	polymer	376	43516.1	2	UniProt (Q8U4M1) Pfam (PF21572) Pfam (PF01798) In PDB	Pyrococcus furiosus
2	B, D	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	234	26760.8	2	UniProt (Q8U4M2) Pfam (PF01269) In PDB	Pyrococcus furiosus

Sequence modifications

A, C: 5 - 369 (UniProt: Q8U4M1)

PDB	External Database	Details
Met -5	-	cloning artifact
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Val 2	-	cloning artifact
Met 3	-	cloning artifact
Ile 4	-	cloning artifact

B, D: 1 - 227 (UniProt: Q8U4M2)

PDB	External Database	Details
Met -6	-	cloning artifact
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	140553.9
All*	Total formula weight	140553.9

*Water molecules are not included.