2M86

Solution structure of Hdm2 with engineered cyclotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	MCo-PMI	polymer	51	5291.0	1		synthetic construct
2	B	E3 ubiquitin-protein ligase Mdm2	polymer	129	14706.7	1	UniProt (Q00987) Pfam (PF02201) In PDB	Homo sapiens (human)	Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

Sequence modifications

B: 82 - 190 (UniProt: Q00987)

PDB	External Database	Details
Gly 62	-	expression tag
Ser 63	-	expression tag
Ser 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag
Ser 71	-	expression tag
Ser 72	-	expression tag
Gly 73	-	expression tag
Leu 74	-	expression tag
Val 75	-	expression tag
Pro 76	-	expression tag
Arg 77	-	expression tag
Gly 78	-	expression tag
Ser 79	-	expression tag
His 80	-	expression tag
Met 81	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	19997.7
All*	Total formula weight	19997.7

*Water molecules are not included.