2KDI

Solution structure of a Ubiquitin/UIM fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin, Vacuolar protein sorting-associated protein 27 fusion protein	polymer	114	12867.4	1	UniProt (B3LRG8) UniProt (P40343) Pfam (PF02809)	Saccharomyces cerevisiae (yeast)	Golgi retention defective protein 11

Sequence modifications

A: 11 - 85 (UniProt: B3LRG8)

PDB	External Database	Details
Met 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Glu 9	-	expression tag
Phe 10	-	expression tag
Ala 14	Val 5	engineered mutation
Trp 54	Phe 45	engineered mutation
Arg 72	Lys 63	engineered mutation
Ser 86	-	linker
Met 87	-	linker
Gly 88	-	linker
Gly 89	-	linker
Ala 90	-	linker
Ala 91	-	linker

A: 92 - 111 (UniProt: P40343)

PDB	External Database	Details
Gly 112	-	expression tag
Gly 113	-	expression tag
Tyr 114	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	12867.4
All*	Total formula weight	12867.4

*Water molecules are not included.