2KBT

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Proto-oncogene vav,Immunoglobulin G-binding protein G	polymer	142	15974.5	1	UniProt (P27870) UniProt (P06654) Pfam (PF00018) Pfam (PF01378)	Mus musculus (house mouse)	p95vav,IgG-binding protein G

Sequence modifications

A: 784 - 844 (UniProt: P27870)

PDB	External Database	Details
Gly 780	-	expression tag
Pro 781	-	expression tag
Gly 782	-	expression tag
Thr 783	-	expression tag
Leu 845	-	linker
Pro 846	-	linker
Glu 847	-	linker
Thr 848	-	linker
Gly 849	-	linker
Gly 850	-	linker
Gly 851	-	linker
Ser 852	-	linker
Gly 853	-	linker
Ser 854	-	linker
Ser 855	-	linker
Met 856	-	linker

A: 857 - 911 (UniProt: P06654)

PDB	External Database	Details
His 912	-	expression tag
Ser 913	-	expression tag
Leu 914	-	expression tag
Glu 915	-	expression tag
His 916	-	expression tag
His 917	-	expression tag
His 918	-	expression tag
His 919	-	expression tag
His 920	-	expression tag
His 921	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	15974.5
All*	Total formula weight	15974.5

*Water molecules are not included.