2J9U

2 Angstrom X-ray structure of the yeast ESCRT-I Vps28 C-terminus in complex with the NZF-N domain from ESCRT-II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28	polymer	96	11151.8	2	UniProt (Q02767) Pfam (PF03997)	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	VPS28
2	B, D (B, D)	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 36	polymer	76	8315.3	2	UniProt (Q06696) Pfam (PF16988)	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	VPS36
3	E, F (B, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H, I, J (A, B, C, D)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, C: 148 - 242 (UniProt: Q02767)

PDB	External Database	Details
Met 147	-	expression tag

B, D: 110 - 171 (UniProt: Q06696)

PDB	External Database	Details
Met 101	-	expression tag
Ala 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
His 106	-	expression tag
His 107	-	expression tag
His 108	-	expression tag
Met 109	-	expression tag
Arg 172	-	expression tag
Asn 173	-	expression tag
Gln 174	-	expression tag
Phe 175	-	expression tag
Gly 176	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	38934.3
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	39065.1

*Water molecules are not included.