2J9O

Structure of PBP-A, L158E mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	TLL2115 PROTEIN	polymer	298	32820.3	4	UniProt (Q8DH45) Pfam (PF13354)	SYNECHOCOCCUS ELONGATUS	SERINE-TYPE CARBOXYPEPTIDASE FROM THERMOSYNECHOCOCCUS ELONGATUS
2	E, F, G (B, C, D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	H, I, J, K (A, B, C, D)	water	water		18.0	1527	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 277 (UniProt: Q8DH45)

PDB	External Database	Details
Met 1	-	expression tag
Glu 278	-	expression tag
Gln 279	-	expression tag
Lys 280	-	expression tag
Leu 281	-	expression tag
Ile 282	-	expression tag
Ser 283	-	expression tag
Glu 284	-	expression tag
Glu 285	-	expression tag
Asp 286	-	expression tag
Leu 287	-	expression tag
Asn 288	-	expression tag
Ser 289	-	expression tag
Ala 290	-	expression tag
Val 291	-	expression tag
Asp 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
Glu 158	Leu 249	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	131281.1
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	131557.4

*Water molecules are not included.