2IIF

single chain Integration Host Factor mutant protein (scIHF2-K45aE) in complex with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Phage P H' site	polymer	35	10802.0	1	PDB (2IIF)	synthetic construct
2	B (D)	DNA (5'-D(*DGP*DGP*DCP*DCP*DAP*DAP*DAP*DAP*DAP*DAP*DGP*DCP*DAP*DTP*DT)-3')	polymer	15	4611.0	1	PDB (2IIF)	synthetic construct
3	C (E)	DNA (5'-D(*DGP*DCP*DTP*DTP*DAP*DTP*DCP*DAP*DAP*DTP*DTP*DTP*DGP*DTP*DTP*DGP*DCP*DAP*DCP*DC)-3')	polymer	20	6074.9	1	PDB (2IIF)	synthetic construct
4	D (A)	Integration host factor	polymer	204	22884.8	1	UniProt (P0A6X7) UniProt (P0A6Y1) Pfam (PF00216)	Escherichia coli	IHF-alpha, IHF-beta
5	E, F, G, H, I... (D, E, A)	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
6	M, N, O, P (C, D, E, A)	water	water		18.0	163	Chemie (HOH)

Sequence modifications

A: 47 - 138 (UniProt: P0A6X7)

PDB	External Database	Details
Glu 89	Lys 45	engineered mutation

A: 4 - 41 (UniProt: P0A6Y1)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Ser 3	-	expression tag

A: 141 - 195 (UniProt: P0A6Y1)

PDB	External Database	Details
Gly 42	-	linker
Gly 43	-	linker
Ser 44	-	linker
Gly 45	-	linker
Gly 46	-	linker
Gly 139	-	linker
Gly 140	-	linker
Gly 196	-	expression tag
Ser 197	-	expression tag
Gly 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	44372.8
Non-Polymers*	Number of molecules	8
	Total formula weight	439.5
All*	Total formula weight	44812.3

*Water molecules are not included.