2I5V

Crystal structure of OspA mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	O	Outer surface protein A	polymer	251	26485.8	1	UniProt (Q45040) Pfam (PF00820) UniProt (by SIFTS) (P0CL66) In PDB	Borrelia burgdorferi (Lyme disease spirochete)
2	O	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
3		water	water		18.0	378	Chemie (HOH)

Sequence modifications

O: 27 - 273 (UniProt: Q45040)

PDB	External Database	Details
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Ser 37	Glu 37	engineered mutation
Ser 45	Glu 45	engineered mutation
Ser 46	Lys 46	engineered mutation
Ala 48	Lys 48	engineered mutation
Ala 60	Lys 60	engineered mutation
Ser 64	Lys 64	engineered mutation
Ala 83	Lys 83	engineered mutation
Ser 104	Glu 104	engineered mutation
Ser 107	Lys 107	engineered mutation
Ile 120	Ser 120	engineered mutation
Ile 121	Ser 121	engineered mutation
Ile 122	Thr 122	engineered mutation
Ile 123	Glu 123	engineered mutation
Ile 124	Glu 124	engineered mutation
Ser 239	Lys 239	engineered mutation
Ser 240	Glu 240	engineered mutation
Ser 254	Lys 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26485.8
Non-Polymers*	Number of molecules	2
	Total formula weight	388.5
All*	Total formula weight	26874.3

*Water molecules are not included.