2HZS

Structure of the Mediator head submodule Med8C/18/20

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	RNA polymerase II mediator complex subunit 20	polymer	209	22786.9	4	UniProt (P34162) Pfam (PF08612)	Saccharomyces cerevisiae (baker's yeast)	Suppressor of RNA polymerase B 2, Hyper-recombination suppressor protein 2
2	B, D, F, H (B, D, F, H)	RNA polymerase II mediator complex subunit 18	polymer	306	34213.4	4	UniProt (P32585) Pfam (PF09637)	Saccharomyces cerevisiae (baker's yeast)	Suppressor of RNA polymerase B 5
3	I, J, K, L (I, J, K, L)	RNA polymerase II mediator complex subunit 8	polymer	27	3217.6	4	UniProt (P38304)	Saccharomyces cerevisiae (baker's yeast)	RNA polymerase II transcriptional regulation mediator 8
4	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	85	Chemie (HOH)

Sequence modifications

B, D, F, H: 2 - 307 (UniProt: P32585)

PDB	External Database	Details
Val 274	Ala 274	engineered mutation

I, J, K, L: 190 - 210 (UniProt: P38304)

PDB	External Database	Details
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	240871.4
All*	Total formula weight	240871.4

*Water molecules are not included.