2HW9

Crystal structure of Lys12Cys/Cys117Val mutant of human acidic fibroblast Growth factor at 1.60 angstrom resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Heparin-binding growth factor 1	polymer	146	16656.7	2	UniProt (P05230) Pfam (PF00167)	Homo sapiens (human)	HBGF-1, Acidic fibroblast growth factor, aFGF, Beta-endothelial cell growth factor, ECGF-beta
2	C, E (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D, F, G (A, B)	FORMIC ACID	non-polymer		46.0	3	Chemie (FMT)
4	H, I (A, B)	water	water		18.0	243	Chemie (HOH)

Sequence modifications

A, B: 1 - 140 (UniProt: P05230)

PDB	External Database	Details
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
Cys 12	Lys 27	engineered mutation
Val 117	Cys 132	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	33313.4
Non-Polymers*	Number of molecules	5
	Total formula weight	330.2
All*	Total formula weight	33643.6

*Water molecules are not included.