2HS8

Crystal structure of the Y364F mutant of 12-oxophytodienoate reductase 3 from tomato

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	12-oxophytodienoate reductase 3	polymer	402	44385.2	2	UniProt (Q9FEW9) Pfam (PF00724)	Solanum lycopersicum	12-oxophytodienoate-10,11-reductase 3, OPDA-reductase 3, LeOPR3
2	C, D (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	E, F (A, B)	water	water		18.0	626	Chemie (HOH)

Sequence modifications

A, B: 1 - 396 (UniProt: Q9FEW9)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 364	Tyr 364	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88770.4
Non-Polymers*	Number of molecules	2
	Total formula weight	912.7
All*	Total formula weight	89683.1

*Water molecules are not included.