2HRS
Crystal structure of L42H V224H design intermediate for GFP metal ion reporter
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Green fluorescent protein | polymer | 238 | 26828.1 | 1 | UniProt (P42212) Pfam (PF01353) In PDB | Aequorea victoria | |
2 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
4 | water | water | 18.0 | 337 | Chemie (HOH) |
Sequence modifications
A: 1 - 238 (UniProt: P42212)
PDB | External Database | Details |
---|---|---|
His 42 | Leu 42 | engineered mutation |
Leu 64 | Phe 64 | engineered mutation |
Cro 66 | Ser 65 | chromophore |
Cro 66 | Tyr 66 | chromophore |
Cro 66 | Gly 67 | chromophore |
Ser 99 | Phe 99 | engineered mutation |
Thr 153 | Met 153 | engineered mutation |
Ala 163 | Val 163 | engineered mutation |
His 224 | Val 224 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 26828.1 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 86.4 | |
All* | Total formula weight | 26914.4 |