2HJO
Crystal structure of V224H design intermediate for GFP metal ion reporter
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green Fluorescent Protein | polymer | 238 | 26921.3 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 2 | B (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 3 | C (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 4 | D (A) | water | water | 18.0 | 285 | Chemie (HOH) |
Sequence modifications
A: 1 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Leu 64 | Phe 64 | engineered mutation |
| Cro 66 | Ser 65 | chromophore |
| Cro 66 | Tyr 66 | chromophore |
| Cro 66 | Gly 67 | chromophore |
| His 224 | Val 224 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 26921.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 86.4 | |
| All* | Total formula weight | 27007.7 |
