2HIM
Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | L-asparaginase 1 | polymer | 358 | 39305.4 | 4 | UniProt (P0A962) Pfam (PF00710) Pfam (PF17763) In PDB | Escherichia coli | L-asparaginase I, L-asparagine amidohydrolase I, L-ASNase I |
| 2 | A, B, C, D | ASPARTIC ACID | non-polymer | 133.1 | 4 | Chemie (ASP) | |||
| 3 | A, B, C, D | ASPARAGINE | non-polymer | 132.1 | 8 | Chemie (ASN) | |||
| 4 | A, B, C, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 8 | Chemie (EDO) | |||
| 5 | water | water | 18.0 | 515 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 338 (UniProt: P0A962)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | cloning artifact |
| Gly -18 | - | cloning artifact |
| Ser -17 | - | cloning artifact |
| Ser -16 | - | cloning artifact |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | cloning artifact |
| Ser -8 | - | cloning artifact |
| Gly -7 | - | cloning artifact |
| Leu -6 | - | cloning artifact |
| Val -5 | - | cloning artifact |
| Pro -4 | - | cloning artifact |
| Arg -3 | - | cloning artifact |
| Gly -2 | - | cloning artifact |
| Ser -1 | - | cloning artifact |
| His 0 | - | cloning artifact |
| Ala 162 | Thr 162 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 157221.7 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 2085.9 | |
| All* | Total formula weight | 159307.6 |
