2HDI

Crystal structure of the Colicin I receptor Cir from E.coli in complex with receptor binding domain of Colicin Ia.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Colicin I receptor	polymer	639	71294.2	1	UniProt (P17315) Pfam (PF07715) Pfam (PF00593)	Escherichia coli
2	B (B)	Colicin-Ia	polymer	113	12891.4	1	UniProt (P06716) Pfam (PF11504)	Escherichia coli
3	C, D (A)	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	2	Chemie (LDA)
4	E, F (A, B)	water	water		18.0	255	Chemie (HOH)

Sequence modifications

A: 26 - 663 (UniProt: P17315)

PDB	External Database	Details
Ser 25	-	cloning artifact
Met 338	Trp 338	engineered mutation
Met 343	Leu 343	engineered mutation
Met 589	Phe 589	engineered mutation
Met 591	Val 591	engineered mutation

B: 282 - 385 (UniProt: P06716)

PDB	External Database	Details
Met 273	-	cloning artifact
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84185.6
Non-Polymers*	Number of molecules	2
	Total formula weight	458.8
All*	Total formula weight	84644.4

*Water molecules are not included.