2GNJ
PKA three fold mutant model of Rho-kinase with Y-27632
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | cAMP-dependent protein kinase, alpha-catalytic subunit | polymer | 350 | 40677.4 | 1 | UniProt (P00517) Pfam (PF00069) | Bos taurus (cattle) | PKA C-alpha |
| 2 | B (I) | cAMP-dependent protein kinase inhibitor alpha | polymer | 20 | 2226.4 | 1 | UniProt (P61926) UniProt (by SIFTS) (Q3SX13) | Oryctolagus cuniculus | PKI-alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform |
| 3 | C (A) | (R)-TRANS-4-(1-AMINOETHYL)-N-(4-PYRIDYL) CYCLOHEXANECARBOXAMIDE | non-polymer | 247.3 | 1 | Chemie (Y27) | |||
| 4 | D, E (A, I) | water | water | 18.0 | 253 | Chemie (HOH) |
Sequence modifications
A: 1 - 350 (UniProt: P00517)
| PDB | External Database | Details |
|---|---|---|
| Ile 49 | Leu 49 | engineered mutation |
| Lys 181 | Gln 181 | engineered mutation |
| Ala 183 | Thr 183 | engineered mutation |
| Tpo 197 | Thr 197 | modified residue |
| Sep 338 | Ser 338 | modified residue |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 42903.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 247.3 | |
| All* | Total formula weight | 43151.2 |
