2GJ8
Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and K+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | tRNA modification GTPase trmE | polymer | 172 | 18901.5 | 4 | UniProt (P25522) Pfam (PF12631) Pfam (PF01926) | Escherichia coli BL21(DE3) | mnmE |
| 2 | E, J, N, S (A, B, C, D) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 4 | Chemie (ALF) | |||
| 3 | F, K, O, T (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 4 | G, L, P, U (A, B, C, D) | POTASSIUM ION | non-polymer | 39.1 | 4 | Chemie (K) | |||
| 5 | H, Q, V (A, C, D) | SELENIUM ATOM | non-polymer | 79.0 | 3 | Chemie (SE) | |||
| 6 | I, M, R, W (A, B, C, D) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 4 | Chemie (GDP) | |||
| 7 | AA, X, Y, Z (D, A, B, C) | water | water | 18.0 | 585 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 216 - 384 (UniProt: P25522)
| PDB | External Database | Details |
|---|---|---|
| Gly 213 | - | cloning artifact |
| Ser 214 | - | cloning artifact |
| His 215 | - | cloning artifact |
| Mse 217 | Met 217 | modified residue |
| Mse 264 | Met 264 | modified residue |
| Mse 302 | Met 302 | modified residue |
| Mse 346 | Met 346 | modified residue |
| Mse 376 | Met 376 | modified residue |
| Mse 382 | Met 382 | modified residue |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 75606.0 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2675.2 | |
| All* | Total formula weight | 78281.1 |
