2GJ8

Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and K+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	tRNA modification GTPase trmE	polymer	172	18901.5	4	UniProt (P25522) Pfam (PF12631) Pfam (PF01926)	Escherichia coli BL21(DE3)	mnmE
2	E, J, N, S (A, B, C, D)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	4	Chemie (ALF) PubChem (5311325)
3	F, K, O, T (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
4	G, L, P, U (A, B, C, D)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K) PubChem (813)
5	H, Q, V (A, C, D)	SELENIUM ATOM	non-polymer		79.0	3	Chemie (SE) PubChem (6326970)
6	I, M, R, W (A, B, C, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
7	AA, X, Y, Z (D, A, B, C)	water	water		18.0	585	Chemie (HOH)

Sequence modifications

A, B, C, D: 216 - 384 (UniProt: P25522)

PDB	External Database	Details
Gly 213	-	cloning artifact
Ser 214	-	cloning artifact
His 215	-	cloning artifact
Mse 217	Met 217	modified residue
Mse 264	Met 264	modified residue
Mse 302	Met 302	modified residue
Mse 346	Met 346	modified residue
Mse 376	Met 376	modified residue
Mse 382	Met 382	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	75606.0
Non-Polymers*	Number of molecules	19
	Total formula weight	2675.2
All*	Total formula weight	78281.1

*Water molecules are not included.