2GIJ

Q138F HincII bound to cognate DNA GTTAAC and Ca2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (E, F)	5'-D(*GP*CP*CP*GP*GP*TP*TP*AP*AP*CP*CP*GP*GP*C)-3'	polymer	14	4281.8	2
2	C, D (A, B)	Type II restriction enzyme HincII	polymer	257	29839.1	2	UniProt (P17743) Pfam (PF09226)	Haemophilus influenzae	Endonuclease HincII, R.HincII
3	E (F)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	F, G (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	H, I, J, K (E, F, A, B)	water	water		18.0	330	Chemie (HOH)

Sequence modifications

A, B: 2 - 258 (UniProt: P17743)

PDB	External Database	Details
Thr 130	Arg 130	conflict
Phe 138	Gln 138	engineered mutation
Trp 173	Ser 173	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	68241.8
Non-Polymers*	Number of molecules	3
	Total formula weight	103.1
All*	Total formula weight	68344.9

*Water molecules are not included.