2GIH
Q138F HincII bound to cognate DNA GTCGAC and Ca2+
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B (E, F) | 5'-D(*GP*CP*CP*GP*GP*TP*CP*GP*AP*CP*CP*GP*GP*C)-3' | polymer | 14 | 4282.8 | 2 | |||
2 | C, D (A, B) | Type II restriction enzyme HincII | polymer | 257 | 29839.1 | 2 | UniProt (P17743) Pfam (PF09226) | Haemophilus influenzae | Endonuclease HincII, R.HincII |
3 | E, F (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
4 | G, H, I, J (E, F, A, B) | water | water | 18.0 | 363 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 258 (UniProt: P17743)
PDB | External Database | Details |
---|---|---|
Thr 130 | Arg 130 | conflict |
Phe 138 | Gln 138 | engineered mutation |
Trp 173 | Ser 173 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 68243.8 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 80.2 | |
All* | Total formula weight | 68323.9 |