2G49

Crystal structure of human insulin-degrading enzyme in complex with glucagon

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Insulin-degrading enzyme	polymer	990	114715.1	2	UniProt (Q5T5N2) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) UniProt (by SIFTS) (P14735)	Homo sapiens (human)	Insulysin, Insulinase, Insulin protease
2	C, D (C, D)	glucagon preproprotein	polymer	29	3486.8	2	GenBank (4503945) Pfam (PF00123) UniProt (by SIFTS) (P01275)
3	E, F (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
4	G, H, I (A, B, C)	water	water		18.0	695	Chemie (HOH)

Sequence modifications

A, B: 42 - 1019 (UniProt: Q5T5N2)

PDB	External Database	Details
Met 30	-	initiating methionine
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ala 37	-	cloning artifact
Ala 38	-	cloning artifact
Gly 39	-	cloning artifact
Ile 40	-	cloning artifact
Pro 41	-	cloning artifact
Gln 111	Glu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	236403.8
Non-Polymers*	Number of molecules	2
	Total formula weight	176.2
All*	Total formula weight	236580.1

*Water molecules are not included.