2G16
Structure of S65A Y66S GFP variant after backbone fragmentation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein | polymer | 66 | 6930.9 | 1 | UniProt (P42212) | Aequorea victoria | |
| 2 | B (B) | Green fluorescent protein | polymer | 172 | 19818.1 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 3 | C, D (A, B) | water | water | 18.0 | 173 | Chemie (HOH) |
Sequence modifications
A: 2 - 64 (UniProt: P42212)
B: 66 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ala 1 | - | cloning artifact |
| PDB | External Database | Details |
|---|---|---|
| Cwr 66 | Ser 65 | chromophore |
| Cwr 66 | Tyr 66 | chromophore |
| Cwr 66 | Gly 67 | chromophore |
| Ser 99 | Phe 99 | engineered mutation |
| Thr 153 | Met 153 | engineered mutation |
| Ala 163 | Val 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 26749.0 | |
| All* | Total formula weight | 26749.0 |
