2FYC
Crystal structure of the catalytic domain of bovine beta1,4-galactosyltransferase-I in complex with alpha-lactalbumin, Ca and UDP-galactose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Alpha-lactalbumin | polymer | 123 | 14015.8 | 2 | UniProt (P29752) Pfam (PF00062) | Mus musculus (house mouse) | Lactose synthase B protein |
| 2 | B, D (B, D) | beta-1,4-galactosyltransferase | polymer | 286 | 32856.5 | 2 | UniProt (P08037) Pfam (PF13733) Pfam (PF02709) | Bos taurus (cattle) | |
| 3 | E, H, J, L (A, B, C, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 4 | F (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 5 | G, K (B, D) | GALACTOSE-URIDINE-5'-DIPHOSPHATE | non-polymer | 566.3 | 2 | Chemie (GDU) | |||
| 6 | I, M (B, D) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 2 | Chemie (UDP) | |||
| 7 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 765 | Chemie (HOH) |
Sequence modifications
B, D: 130 - 402 (UniProt: P08037)
| PDB | External Database | Details |
|---|---|---|
| Ala 117 | - | cloning artifact |
| Ser 118 | - | cloning artifact |
| Met 119 | - | cloning artifact |
| Thr 120 | - | cloning artifact |
| Gly 121 | - | cloning artifact |
| Gly 122 | - | cloning artifact |
| Gln 123 | - | cloning artifact |
| Gln 124 | - | cloning artifact |
| Met 125 | - | cloning artifact |
| Gly 126 | - | cloning artifact |
| Arg 127 | - | cloning artifact |
| Gly 128 | - | cloning artifact |
| Ser 129 | - | cloning artifact |
| Thr 342 | Cys 269 | engineered mutation |
| His 344 | Met 271 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 93744.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2296.5 | |
| All* | Total formula weight | 96041.2 |
