2FYC
Crystal structure of the catalytic domain of bovine beta1,4-galactosyltransferase-I in complex with alpha-lactalbumin, Ca and UDP-galactose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Alpha-lactalbumin | polymer | 123 | 14015.8 | 2 | UniProt (P29752) Pfam (PF00062) | Mus musculus (house mouse) | Lactose synthase B protein |
| 2 | B, D (B, D) | beta-1,4-galactosyltransferase | polymer | 286 | 32856.5 | 2 | UniProt (P08037) Pfam (PF13733) Pfam (PF02709) | Bos taurus (cattle) | |
| 3 | E, H, J, L (A, B, C, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 4 | F (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) PubChem (4478249) | |||
| 5 | G, K (B, D) | GALACTOSE-URIDINE-5'-DIPHOSPHATE | non-polymer | 566.3 | 2 | Chemie (GDU) | |||
| 6 | I, M (B, D) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 2 | Chemie (UDP) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241) | |||
| 7 | N, O, P, Q (A, B, C, D) | water | water | 18.0 | 765 | Chemie (HOH) |
Sequence modifications
B, D: 130 - 402 (UniProt: P08037)
| PDB | External Database | Details |
|---|---|---|
| Ala 117 | - | cloning artifact |
| Ser 118 | - | cloning artifact |
| Met 119 | - | cloning artifact |
| Thr 120 | - | cloning artifact |
| Gly 121 | - | cloning artifact |
| Gly 122 | - | cloning artifact |
| Gln 123 | - | cloning artifact |
| Gln 124 | - | cloning artifact |
| Met 125 | - | cloning artifact |
| Gly 126 | - | cloning artifact |
| Arg 127 | - | cloning artifact |
| Gly 128 | - | cloning artifact |
| Ser 129 | - | cloning artifact |
| Thr 342 | Cys 269 | engineered mutation |
| His 344 | Met 271 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 93744.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2296.5 | |
| All* | Total formula weight | 96041.2 |
