2FFI

Crystal Structure of Putative 2-Pyrone-4,6-Dicarboxylic Acid Hydrolase from Pseudomonas putida, Northeast Structural Genomics Target PpR23.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	2-pyrone-4,6-dicarboxylic acid hydrolase, putative	polymer	288	32427.7	2	UniProt (Q88M75) Pfam (PF04909)	Pseudomonas putida
2	C, D (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	E, F (A, B)	water	water		18.0	55	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: Q88M75)

PDB	External Database	Details
Mse 1	Met 1	modified residue
Mse 4	Met 4	modified residue
Mse 88	Met 88	modified residue
Mse 101	Met 101	modified residue
Mse 114	Met 114	modified residue
Mse 118	Met 118	modified residue
Mse 232	Met 232	modified residue
Leu 281	-	cloning artifact
Glu 282	-	cloning artifact
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64855.4
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	65045.3

*Water molecules are not included.