2F66

Structure of the ESCRT-I endosomal trafficking complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease	polymer	65	7550.7	2	UniProt (P25604) Pfam (PF09454) In PDB	Saccharomyces cerevisiae (baker's yeast)	Vacuolar protein sorting-associated protein VPS23
2	B, E	Vacuolar protein sorting-associated protein VPS28	polymer	116	13296.8	2	UniProt (Q02767) Pfam (PF03997) In PDB	Saccharomyces cerevisiae (baker's yeast)
3	C, F	Protein SRN2	polymer	82	9833.9	2	UniProt (Q99176) Pfam (PF07200) In PDB	Saccharomyces cerevisiae (baker's yeast)
4	B, C	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5		water	water		18.0	23	Chemie (HOH)

Sequence modifications

A, D: 322 - 385 (UniProt: P25604)

PDB	External Database	Details
Met 321	-	cloning artifact
Ala 344	Cys 344	engineered mutation

B, E: 15 - 125 (UniProt: Q02767)

PDB	External Database	Details
Gly 9	-	cloning artifact
Ala 10	-	cloning artifact
Met 11	-	cloning artifact
Ala 101	Cys 101	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	61362.7
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	61554.8

*Water molecules are not included.