2F54
Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, F (A, F) | HLA class I histocompatibility antigen | polymer | 274 | 31725.1 | 2 | UniProt (P01892) Pfam (PF00129) Pfam (PF07654) UniProt (by SIFTS) (P04439) | Homo sapiens (human) | MHC class I antigen A*2 |
| 2 | B, G (B, G) | Beta-2-microglobulin | polymer | 100 | 11793.3 | 2 | UniProt (P61769) Pfam (PF07654) | Homo sapiens (human) | |
| 3 | C, H (C, H) | Cancer/testis antigen 1B | polymer | 9 | 1094.3 | 2 | UniProt (P78358) | L antigen family member 2, LAGE-2 protein, Autoimmunogenic cancer/testis antigen NY-ESO-1 | |
| 4 | D, I (D, K) | T-cell receptor alpha chain | polymer | 206 | 22439.8 | 2 | UniProt (Q6PIZ8) Pfam (PF09291) UniProt (by SIFTS) (P01848) | Homo sapiens (human) | |
| 5 | E, J (E, L) | T-cell receptor beta chain | polymer | 241 | 27006.0 | 2 | UniProt (Q6NS87) Pfam (PF07654) UniProt (by SIFTS) (P01850) | Homo sapiens (human) | |
| 6 | K, L, M, N, O... (A, B, C, D, E...) | water | water | 18.0 | 30 | Chemie (HOH) |
Sequence modifications
B, G: 1 - 99 (UniProt: P61769)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | cloning artifact |
| Cys 67 | Tyr 87 | engineered mutation |
| Cys 91 | Lys 111 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 188117.0 | |
| All* | Total formula weight | 188117.0 |
