2F53
Directed Evolution of Human T-cell Receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without apparent cross-reactivity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | HLA class I histocompatibility antigen | polymer | 275 | 31854.2 | 1 | UniProt (P01892) Pfam (PF00129) Pfam (PF07654) UniProt (by SIFTS) (P04439) | Homo sapiens (human) | MHC class I antigen A*2 |
| 2 | B (B) | Beta-2-microglobulin | polymer | 100 | 11853.3 | 1 | UniProt (P61769) Pfam (PF07654) | Homo sapiens (human) | |
| 3 | C (C) | Cancer/testis antigen 1B | polymer | 9 | 1094.3 | 1 | UniProt (P78358) | L antigen family member 2, LAGE-2 protein, Autoimmunogenic cancer/testis antigen NY-ESO-1 | |
| 4 | D (D) | T-cell Receptor, alpha chain | polymer | 193 | 21211.6 | 1 | UniProt (Q6PIZ8) Pfam (PF09291) UniProt (by SIFTS) (P01848) | Homo sapiens (human) | |
| 5 | E (E) | T-cell receptor, beta chain | polymer | 243 | 27267.3 | 1 | Homo sapiens (human) | ||
| 6 | F (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | G, H, I, J (A, B, E) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 8 | K, L, M, N, O (A, B, C, D, E) | water | water | 18.0 | 728 | Chemie (HOH) |
Sequence modifications
B: 1 - 99 (UniProt: P61769)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | cloning artifact |
| Cys 91 | Lys 111 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 93280.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 391.4 | |
| All* | Total formula weight | 93672.1 |
