2F2Q
High resolution crystal structure of T4 lysozyme mutant L20R63/A liganded to guanidinium ion
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Lysozyme | polymer | 175 | 19685.5 | 1 | UniProt (P00720) Pfam (PF00959) | Enterobacteria phage T4 | Lysis protein, Muramidase, Endolysin |
| 2 | B (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 3 | C (A) | GUANIDINE | non-polymer | 59.1 | 1 | Chemie (GAI) | |||
| 4 | D (A) | 2-HYDROXYETHYL DISULFIDE | non-polymer | 154.3 | 1 | Chemie (HED) | |||
| 5 | E (A) | water | water | 18.0 | 181 | Chemie (HOH) |
Sequence modifications
A: 1 - 175 (UniProt: P00720)
| PDB | External Database | Details |
|---|---|---|
| Ile 39 | Leu 39 | engineered mutation |
| Lys 48 | 48 | insertion |
| Ala 49 | 49 | insertion |
| Ile 50 | 50 | insertion |
| Asn 51 | 51 | insertion |
| Ala 52 | 52 | insertion |
| Ala 53 | 53 | insertion |
| Lys 54 | 54 | insertion |
| Ser 55 | 55 | insertion |
| Glu 56 | 56 | insertion |
| Leu 57 | 57 | insertion |
| Asp 58 | 58 | insertion |
| Ala 63 | Arg 63 | engineered mutation |
| Thr 65 | Cys 65 | engineered mutation |
| Ala 108 | Cys 108 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 19685.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 248.8 | |
| All* | Total formula weight | 19934.3 |
