2F2Q

High resolution crystal structure of T4 lysozyme mutant L20R63/A liganded to guanidinium ion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lysozyme	polymer	175	19685.5	1	UniProt (P00720) Pfam (PF00959) In PDB	Enterobacteria phage T4	Lysis protein, Muramidase, Endolysin
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	A	GUANIDINE	non-polymer		59.1	1	Chemie (GAI)
4	A	2-HYDROXYETHYL DISULFIDE	non-polymer		154.3	1	Chemie (HED)
5		water	water		18.0	181	Chemie (HOH)

Sequence modifications

A: 1 - 175 (UniProt: P00720)

PDB	External Database	Details
Ile 39	Leu 39	engineered mutation
Lys 48	48	insertion
Ala 49	49	insertion
Ile 50	50	insertion
Asn 51	51	insertion
Ala 52	52	insertion
Ala 53	53	insertion
Lys 54	54	insertion
Ser 55	55	insertion
Glu 56	56	insertion
Leu 57	57	insertion
Asp 58	58	insertion
Ala 63	Arg 63	engineered mutation
Thr 65	Cys 65	engineered mutation
Ala 108	Cys 108	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	19685.5
Non-Polymers*	Number of molecules	3
	Total formula weight	248.8
All*	Total formula weight	19934.3

*Water molecules are not included.