2F03

Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (E, G)	DNA (5'-D(*AP*TP*G*TP*GP*GP*CP*CP*AP*AP*CP*AP*AP*GP*GP*CP*CP*TP*AP*TP*T)-3')	polymer	21	6447.2	2
2	B, D (F, H)	DNA (5'-D(*AP*AP*TP*AP*GP*GP*CP*CP*TP*TP*GP*TP*TP*GP*GP*CP*CP*AP*CP*AP*T)-3')	polymer	21	6438.2	2
3	E, F (A, C)	Type II restriction enzyme SfiI	polymer	269	31273.6	2	UniProt (O52512) Pfam (PF11487)		Endonuclease SfiI, R.SfiI
4	G, H (A, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	I, J, K, L, M (E, F, G, A, C)	water	water		18.0	111	Chemie (HOH)

Sequence modifications

A, C: 1 - 269 (UniProt: O52512)

PDB	External Database	Details
Mse 1	Met 1	modified residue
Mse 119	Met 119	modified residue
Mse 134	Met 134	modified residue
Mse 143	Met 143	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	88317.9
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	88398.0

*Water molecules are not included.