2EZ8
Pyruvate oxidase variant F479W in complex with reaction intermediate 2-lactyl-thiamin diphosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Pyruvate oxidase | polymer | 603 | 66245.8 | 2 | UniProt (P37063) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) | Lactobacillus plantarum | Pyruvic oxidase, POX |
| 2 | C, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D, H (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | E, J (A, B) | 3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-2-(1-CARBOXY-1-HYDROXYETHYL)-5-(2-{[HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM | non-polymer | 513.4 | 2 | Chemie (TDL) | |||
| 5 | F, K (A, B) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
| 6 | G, L (A, B) | PYRUVIC ACID | non-polymer | 88.1 | 2 | Chemie (PYR) | |||
| 7 | M, N (A, B) | water | water | 18.0 | 993 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 603 (UniProt: P37063)
| PDB | External Database | Details |
|---|---|---|
| Trp 479 | Phe 479 | engineered mutation |
| Met 561 | Thr 561 | see remark 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 132491.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2868.6 | |
| All* | Total formula weight | 135360.2 |
